MassIVE MSV000088237

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Integrated proteomic and metabolomic analyses of the mitochondrial neurodegenerative disease MELAS

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Description

MELAS (mitochondrial encephalomyopathy, lactic acidosis, stroke-like episodes) is a progressive neurodegenerative disease caused by pathogenic mitochondrial DNA variants. The pathogenic mechanism of MELAS remains enigmatic due to the exceptional clinical heterogeneity and the obscure genotype-phenotype correlation among MELAS patients. To gain insights into the pathogenic signature of MELAS, we designed a comprehensive strategy integrating proteomics and metabolomics in patient-derived dermal fibroblasts harboring the ultra-rare MELAS pathogenic variant m.14453G>A, specifically affecting the mitochondrial respiratory complex I. Global proteomics was achieved by data-dependent acquisition (DDA) and verified by data-independent acquisition (DIA) using both Spectronaut and the recently launched MaxDIA platforms. Comprehensive metabolite coverage was achieved for both polar and nonpolar metabolites in both reverse phase and HILIC LC-MS/MS analyses. Our proof-of-principle MELAS study with multi-omics integration revealed OXPHOS dysregulation with a predominant deficiency of complex I subunits, as well as alterations in key bioenergetic pathways, glycolysis, tricarboxylic acid cycle, and fatty acid ?-oxidation. The most clinically relevant discovery is the downregulation of the arginine biosynthesis pathway, likely due to blocked argininosuccinate synthase, which is congruent with the MELAS cardinal symptom of stroke-like episodes and its current treatment by arginine infusion. In conclusion, we demonstrated an integrated proteomic and metabolomic strategy for patient-derived fibroblasts, which has great clinical potential to discover therapeutic targets and design personalized interventions after validation with a larger patient cohort in the future. [doi:10.25345/C5756P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: MELAS ; mitochondrial disease ; Proteomics ; Metabolomics

Contact

Principal Investigators:
(in alphabetical order) 		Ling Hao, The George Washington University, United States of America
Submitting User: haolab

Publications

Li H, Uittenbogaard M, Navarro R, Ahmed M, Gropman A, Chiaramello A, Hao L.
Integrated proteomic and metabolomic analyses of the mitochondrial neurodegenerative disease MELAS.
Mol Omics. 2022 Mar 28;18(3):196-205. Epub 2022 Mar 28.

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