MassIVE MSV000099802

Partial Public PXD070501

RPE-Glut1 KO Mouse Retina Proteomics

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Description

9-week post-tamoxifen (PT) mouse retinas from the RPE-Glut1 KO mice. The tryptic digest from 25ug of retina protein from 5 replicates of 2 sample conditions: Control (Glut1fl/fl) and KO (REP65-cre Glut1fl/fl) were analyzed using a 40min LC-MS/MS DIA run on the Thermo Orbitrap Astral mass spectrometer.MS data were searched against a spectral library generated from the UniProt mouse database (08/21/2023) and a contaminant database using DIA-NN v1.8.1. Mass spectrometry analysis was performed at The Wistar Institute. [doi:10.25345/C5FN11561] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: GLUT1 ; Tamoxifen ; Retina ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Nancy Philp, Thomas Jefferson University, USA
Submitting User: Anneliese
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.