MassIVE MSV000096415

Complete Public PXD057893

Distinguishing N-Terminal Methylation from Near-isobaric Modifications by Statistical Analysis of Mass Error Distributions of Fragment Ions

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Description

alpha-N-terminal methylation is an understudied post-translational modification with presumed functions involving protein-protein and/or protein-DNA interaction. The covalent addition of mono-, di-, trimethyl groups to free alpha-amino group has proven to be difficult to profile globally by mass spectrometry due to trace endogenous amount and interference from near-isobaric modifications such as Nt-acetylation, even after N-terminome enrichment. To address this problem, we assume that in each MS2 spectrum b-ions will have a different mass error distribution compared to y-ions if the spectrum is falsely assigned to near-isobaric Nt-modification and exploit this statistically to correct the Nt-modification, a procedure we name as mass error test (MET) [doi:10.25345/C5QB9VH99] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: N-terminal methylation ; mass error test ; post-translational modification ; mass error distribution ; near isobaric modification ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Cheolju lee, Korea Institute of Science and Technology, south korea
Submitting User: hanklee321

Publications

Lee H, Ju S, Lee S, Lee SH, Kim SY, Park N, Hwang CS, Park KS, Lee C.
Distinguishing N-Terminal Methylation from Near-Isobaric Modifications by Statistical Analysis of Mass Error Distributions of Fragment Ions.
J Proteome Res. Epub 2025 Jul 30.

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