MassIVE MSV000093213

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The modified RNA base acp3U is an attachment site for N-glycans in glycoRNA

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Description

GlycoRNA consists of RNAs modified with secretory N-glycans that are presented on the cell surface. While previous work supported a covalent linkage between RNA and glycans, the direct chemical nature of the RNA-glycan connection was not described. Here we develop a sensitive and scalable protocol to detect and characterize native glycoRNAs. Leveraging periodate oxidation and aldehyde ligation (rPAL) and Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS), we identified the modified RNA base 3-(3-amino-3-carboxypropyl)uridine (acp3U) as a site of attachment of N-glycans in glycoRNA. rPAL offers sensitivity and robustness as an approach for characterizing direct glycan-RNA linkages occurring in cells, and its flexibility will enable further exploration of glycoRNA biology. [doi:10.25345/C52Z1309R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: RNA ; glycoRNA ; glycosylation ; epitranscriptomics

Contact

Principal Investigators:
(in alphabetical order) 		Ryan A. Flynn, Boston Children's Hospital, United States
Benjamin A. Garcia, Washington University School of Medicine in St. Louis, United States
Submitting User: xie753951

Publications

Xie Y, Chai P, Till NA, Hemberger H, Lebedenko CG, Porat J, Watkins CP, Caldwell RM, George BM, Perr J, Bertozzi CR, Garcia BA, Flynn RA.
The modified RNA base acp3U is an attachment site for N-glycans in glycoRNA.
Cell. 2024 Sep 19;187(19):5228-5237.e12. Epub 2024 Aug 21.

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