Description
Arabidopsis thaliana (thale cress) wild-type (WT) and transgenic lines were in the Columbia-0 (Col-0) background. The T-DNA insertion mutants of AOP3 (SALK_001655C), BZO1 (two independent lines, SALK_094196 and GABI_565B09) and SCPL17 (two lines independent, SALK_075481 and SALK_126264) and the WT were sowed directly in fertilized soil. Plants were grown in optimal conditions (control) in a growth chamber with long day photoperiod (16h light/21 celsius degrees - 8h dark/19 celsius degrees). Watering was made with water. At the onset of flowering (occurring between 5 and 6 weeks after seed sowing), high temperature (HT) was applied to half of the plants of the corresponding experiment by transferring them to another growth chamber (SANYO, Thermo Fisher Scientific) displaying high temperature conditions (16h of light/27 celsius degrees - 8h of dark/24 celsius degrees). The other half of the plants, constituting control plants, remained in the initial growth chamber with control temperature conditions. Dry seeds were harvested. Untargeted metabolomics (LC-MS/MS) were performed with those samples of dry seeds.
[doi:10.25345/C5TT4G626]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: High temperature ; Seeds ; Glucosinolates ; Arabidopsis thaliana ; Seed development ; DatasetType:Metabolomics
Contact
Principal Investigators:
(in alphabetical order)
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Massimiliano CORSO, Institut Jean-Pierre Bourgin (INRAE), France
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macorso
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Number of distinct conditions across all analyses (original submission and reanalyses)
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Distinct condition labels are counted across all files submitted in the "Metadata" category
having a "Condition" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses)
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Distinct replicate labels are counted across all files submitted in the "Metadata" category
having a "BioReplicate" or "Replicate" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses)
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The technical replicate count is defined as the maximum number of times any one distinct
combination of condition and biological replicate was analyzed across all files submitted in the
"Metadata" category. In the case of fractionated experiments, only the first fraction is
considered.
"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original
submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported
across all analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.