Description
Streptomyces cinnamoneus NRRL B-24434 and Streptomyces aureoverticillatus NRRL B-3326 were cultivated in ISP2 liquid medium for 7 days at 28C. This was followed by n-butanol liquid:liquid extraction of the growth medium, rotovaporation, resuspension in 80% methanol, filtration with Whatman 0.2 micron syringeless filters followed by LC-MS/MS analysis. LC-MS data was collected on a Thermo QExactive Orbitrap connected to a Vanquish LC system. LC settings were as follows: injection volume 5 microliter, Phenomenex Kinetex 2.6 micrometer C18 reverse phase 100 A 150x3 mm LC column, LC gradient, solvent A, 0.1% formic acid, solvent B, acetonitrile (0.1% formic acid), 0 min, 10% B, 5 min, 60% B, 5.1 min, 95% B, 6 min, 95% B, 6.1 min, 10% B, 9.9 min, 10% B, 0.5 ml/min. MS settings were as follows - positive ion mode, full MS, resolution 70000, mass range 400-1200 m/z, dd-MS2 (data-dependent MS/MS), resolution 17500, AGC target 1x10^5, loop count 5, isolation width 1.0 m/z, collision energy 25 eV, dynamic exclusion 0.5 s.
[doi:10.25345/C5862BP2W]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: polyketide, monazomycin, oasomycin, seq2pks
Contact
Principal Investigators:
(in alphabetical order)
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Roland Kersten, University of Michigan, Ann Arbor, United States
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| Submitting User: |
Rolandoo1
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Conditions:
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Biological Replicates:
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Technical Replicates:
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| Identification Results |
Proteins (Human, Remapped):
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Proteins (Reported):
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Peptides:
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Variant Peptides:
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PSMs:
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Differential Proteins:
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Quantified Proteins:
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GNPS content goes here (MSV000094063 [task=cd536d58ccb74ae6a2f33b4a8ee727f3])
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link to the right).
Number of distinct conditions across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct condition labels are counted across all files submitted in the "Metadata" category
having a "Condition" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct replicate labels are counted across all files submitted in the "Metadata" category
having a "BioReplicate" or "Replicate" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses)
associated with this dataset.
The technical replicate count is defined as the maximum number of times any one distinct
combination of condition and biological replicate was analyzed across all files submitted in the
"Metadata" category. In the case of fractionated experiments, only the first fraction is
considered.
"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original
submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported
across all analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.