MassIVE MSV000097724

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Elucidating the role of bacteriophage lifestyle strategy in Microbially-Mediated Perennial Rhizosphere Nitrogen Transformations

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Description

Illumination of the interplay between viral community lifestyle strategy (lytic vs. temperate) and the impact of these interactions on microbial population dynamics and metabolic functioning in soil is critical to our understanding of bioenergy crop productivity as well as carbon and nitrogen cycling more broadly. We will leverage an existing set of samples from switchgrass growing on marginal lands that were collected in a 2-week interval time-series over two years with comprehensive data on soil nitrogen pools, plant growth, and existing 16S-focused data on rhizosphere bacteria. We will investigate the role of phage in microbiome dynamics and nitrogen cycling in this system. Ecological and evolutionary trade-offs for bacteria and their bacteriophage are predicted to lead to shifts in viral lifestyle over the growing season and in response to shifts in nutrient availability. In particular, lytic phage are hypothesized to be more dominant during periods of rapid bacterial growth. Furthermore, temperate phage are hypothesized to be triggered into lysis by root exudates or microbial metabolites in soil. Lysis events are predicted to underlie variation in biogeochemical cycling, since the death of bacteria through viral lysis will liberate carbon and nitrogen compounds, contributing to the fluctuations in these compounds that our temporal dataset has documented. The work (proposal:https://doi.org/10.46936/10.25585/60001368) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231. [doi:10.25345/C58K7585K] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: viral community ; rhizosphere bacteria ; nitrogen cycling ; DatasetType:Metabolomics

Contact

Principal Investigators:
(in alphabetical order) 		Rick White, University of North Carolina Charlotte, United States
Submitting User: bpbowen
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