MassIVE MSV000096582

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GNPS - Population wide metabolomic analysis identifies peptide polymers as markers of coral stress independent of genotype

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Description

Effective coral conservation depends on understanding how stress impacts reef biology and health. Coral bleaching varies widely across individuals, yet its dependence on the genetic background of coral animals is poorly understood. Given this knowledge gap, we strove to identify genotype-independent metabolite markers of stress in two Hawaiian coral species, Montipora capitata and Pocillopora acuta. After exposure to thermal and pH stress, we used untargeted metabolomic profiling to investigate the physiological response of different coral genotypes (and ploidies) of each species. Our previous studies showed that dipeptides correlate with stress, and we found these metabolites showed a consistent response across different genotypes and ploidies. We further explored dark metabolites that are stress-responsive, and our tandem mass spectrometry (LC-MS2) results suggest that these candidates are larger tri- and tetrapeptides. Our results identify short peptides as critical intermediates in the coral stress response which provide diagnostic biomarkers for field applications. [doi:10.25345/C5D50G886] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Dipeptides ; Tripeptides ; Tetrapeptides ; Stress response ; Holobiont ; Montipora capitata ; Pocillopora acuta ; DatasetType:Metabolomics

Contact

Principal Investigators:
(in alphabetical order) 		Xiaoyang Su, Rutgers Cancer Institute of New Jersey, USA
Submitting User: enchiles
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GNPS content goes here (MSV000096582 [task=cf242b11e0114fe4aab2a8bddc51d00d])
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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