MassIVE MSV000092700

Partial Public

GNPS - Parallel host and microbial pathways regulate lipid desaturation

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Description

4 datasets, each in their own subfolder. 1) acdh-11 comparative metabolomics. 4 genotypes (N2, fcmt-1, WT FAT-7::GFP, and acdh-11;FAT-7::GFP) grown at 3 temperatures (15, 20, 25). Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately. 2) acdh-11 comparative metabolomics re: diet. 2 genotypes (WT FAT-7::GFP, and acdh-11;FAT-7::GFP) and 2 diets (WT E. coli, BW25113, and cyclopropane-deficient E. Coli, JW1653-1). The cyclopropane-deficient strain was additionally supplemented with cyclopropane fatty acids lactobacillic acid (LBA) or dihydrosterculic acid (DHSA) at 20 uM. 3) fcmt-1 comparative metabolomics. 4 genotypes (N2, fcmt-1(gk155709), fcmt-1(tm2382), hacl-1(tm6725)) grown at 20C. Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately. 4) vaccenic acid isotope labeling. 2 genotypes (N2 and hacl-1(tm6725)) grown at 20C. Each genotype was supplemented with vehicle, cis-vaccenic acid (cis-VA), stable isotope labeled D13-cis-VA, trans-vaccenic acid (trans-VA), and stable isotope labeled D13-trans-VA. Conditioned media (exo-metabolome) and worm pellet (endo-metabolome) isolated and extracted separately. [doi:10.25345/C50R9MF18] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Metabolomics ; C. elegans ; Fatty acid ; Cyclopropane

Contact

Principal Investigators:
(in alphabetical order) 		Frank Schroeder, Boyce Thompson Institute, Cornell University, USA
Submitting User: bwf7

Publications

Fox BW, Helf MJ, Burkhardt RN, Artyukhin AB, Curtis BJ, Palomino DF, Schroeder AF, Chaturbedi A, Tauffenberger A, Wrobel CJJ, Zhang YK, Lee SS, Schroeder FC.
Evolutionarily related host and microbial pathways regulate fat desaturation in C. elegans.
Nat Commun. 2024 Feb 19;15(1):1520. Epub 2024 Feb 19.

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GNPS content goes here (MSV000092700 [task=cf8b04dcd6c84828b8b6c74e008b3239])
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