MassIVE MSV000087475

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Overexpression of griseusins in Streptomyces sp. CA-256286

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Description

Employing multi-omics methods for overexpression of griseusins in Streptomyces sp. CA-25628. This set of data proves that the introduction of the SARPs regulator activates the production of griseusins ( [M+H]+= 749.2589, 586.2288, 796.2771), the inactivation of the griseusin expression using CRISPR-cBEST base editing, and the heterologous expression of the BGC responsible for their production, complemented with the SARPs regulator, activates their production in S. albus J1074. [doi:10.25345/C5M817] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Expression of novel compounds ; Regulators ; Griseusins ; Streptomyces sp. CA-256286 ; CRISPRc-BEST Inactivations

Contact

Principal Investigators:
(in alphabetical order) 		Karl Tilmann Weber, DTU, Denmark
Submitting User: eeko

Publications

Charlotte Beck, Tetiana Gren, Tue S. Jørgensen, Ignacio González, Fernando Román-Hurtado, Daniel Oves-Costales, Olga Genilloud, and Tilmann Weber.
Complete Genome Sequence of Streptomyces sp. Strain CA-256286.
Microbiology Resource Announcements Vol. 10, No. 22; DOI: 10.1128/MRA.00290-21.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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