In this project we report 133 biologically distinct mouse liver cell (Hepa 1-6 and FL83B) and mouse tissue (brain, kidney, and liver) phosphoproteomes prepared using a newly developed scalable, single-run phosphoproteomics platform, using titanium dioxide (TiO2) based phosphopeptide enrichment and performed in 96-well format. We combine this technology with in situ hepatic stimulation, to generate time-resolved maps of insulin signaling in the mouse liver.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Mouse ; Liver ; Kidney ; Brain ; Q Exactive ; Phosphoproteome
Principal Investigators: (in alphabetical order) |
Matthias Mann, Dept. Proteomics and Signal Transduction Max-Planck Institute of Biochemistry, N/A |
Submitting User: | ccms |
Humphrey SJ, Azimifar SB, Mann M.
High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics.
Nat. Biotechnol. 2015 Sep;33(9):990-5. Epub 2015 Aug 17.
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