We isolated pgRNA and characterized its protein interactome in cells transfected with packaging competent and packaging incompetent HBV plasmids to identify proteins potentially playing a role in viral packaging. We identified over 250 proteins preferentially associated with pgRNA from the packaging competent version of the virus. These included proteins known to support capsid formation, enhance viral gene expression, catalyze nucleocapsid dephosphorylation, and bind to N6-methylations on the viral genome.
[doi:10.25345/C5FQ9QH0M]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: interactomics ; RNA binding proteins ; Hepatitis B Virus ; viral packaging ; HyPR-MS ; RNA-protein interactions ; host-pathogen interactions ; pregenomic RNA
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Lloyd Smith, University of Wisconsin-Madison, United States |
Submitting User: | iwhitworth |
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Owner | Reanalyses | |
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