The faster sequencing speed available on the latest Q Exactive HF mass spectrometer is investigated and evaluated by four different acquisition methods and benchmarked across three generations of Q Exactive instruments. Also the instrument capabilities for offline high pH reversed-phase peptide fractionation and single-shot phosphoproteomics are evaluated.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Orbitrap. Q Exactive HF. Shotgun proteomics. HCD. HeLa. Phosphoproteomics. Single-shot analysis. High pH Reversed-phase fractionation. Parallel acquisition. Deep proteome coverage
Principal Investigators: (in alphabetical order) |
Dr Jesper V. Olsen |
Submitting User: | ccms |
Kelstrup CD, Jersie-Christensen RR, Batth TS, Arrey TN, Kuehn A, Kellmann M, Olsen JV.
Rapid and deep proteomes by faster sequencing on a benchtop quadrupole ultra-high-field Orbitrap mass spectrometer.
J. Proteome Res. 2014 Dec 5;13(12):6187-95. Epub 2014 Nov 10.
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Experimental Design | ||
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Identification Results | ||
Proteins (Human, Remapped):
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Proteins (Reported):
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Peptides:
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PSMs:
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Quantification Results | ||
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