MassIVE MSV000083404

Partial Public PXD012599

Three-pronged 'Pitchfork' strategy enables an extensive description of human membrane proteome and identification of missing proteins

Description

In this study, we combined the transmembrane segment-oriented method hpTC with a solid phase enrichment of glycopeptides (SPEG) and standard 'detergent and trypsin' approach into a three-pronged 'Pitchfork' strategy to maximize membrane proteome coverage. The strategy enabled identification of over 1200 IMPs in human lymphoma cells. [doi:10.25345/C5GS49] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: integral membrane proteins ; cyanogen bromide ; glycopeptide enrichment ; missing proteins ; mantle cell lymphoma

Contact

Principal Investigators: Ondrej Vit, Charles University, First Faculty of Medicine, Czech Republic
Submitting User: hwllffrdd
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.
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