MassIVE MSV000098290

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GNPS - Heat sensitivity in the Antarctic green alga Chlamydomonas priscui

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Description

Chlamydomonas priscui (previously sp. UWO241) is a green alga isolated from the ice-covered Antarctic Lake Bonney, where it thrives in a permanently cold, low light and hypersaline environment. We propose a multi-omics approach to investigate how this obligate cold extremophile (psychrophile) responds to high temperature stress stress. We will examine the role of salinity and light intensity on temperature resilience in C. priscui. We have shown that C. priscuii acclimated to high salinity and low light (HS-LL; similar to its natural habitat) exhibits significantly slower rates of heat-induced cell death, when compared to cultures acclimated to predicted climate change conditions accompanied with increased light or decreased salinity. We will examine the molecular basis of this response. Algae will be grown photoautotrophically at 4°C with continuous light to mimic the polar day and acclimated to the four defined conditions, adjusting salinity and light. Mid-log phase cultures will be exposed to short-term stress at 24°C for 12h, followed by 24h recovery at 4°C. The work (proposal:https://doi.org/10.46936/10.25585/60008464) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231. [doi:10.25345/C5C824T0V] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: heat stress ; multi-omics ; Antarctic ; Lake Bonney ; salinity ; lipidomics ; DatasetType:Metabolomics

Contact

Principal Investigators:
(in alphabetical order) 		Marina Cvetkovska, U of Ottawa, United States
Submitting User: bpbowen
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GNPS content goes here (MSV000098290 [task=d3564affe2664a369369856845eb63a4])
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Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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