MassIVE MSV000083636

Partial Public PXD013272

Paenibacillus larvae exoproteins of ERIC I-IV genotypes

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Description

The following strains of Paenibacillus larvae classified as ERIC I-IV genotypes (pl1-pl4) were used in the study: ERIC I - DSM-7030; ERIC II - DSM-25430; ERIC III - LMG-16252; and ERIC IV - DSM-3615. The 7 biological replicates (a-g) of the exoprotein fractions prepared from each ERIC genotype were used in the nanoLC-MS/MS analysis. All 28 samples were consecutively analyzed using an OrbitrapTM FusionTM Tribrid q-OT-IT mass spectrometer. To improve the protein identification and easy exclude contaminants from the medium, we considered the proteins that could originate from the yeast extract and Mueller-Hinton broth (contains beef fusion and casein peptone-acidic hydrolysate) in analyzing the MS data. Thus, we included the sequences related to Saccharomyces cerevisiae and Bos taurus, respectively, to the list of contaminants in MaxQuant. The contaminants.fasta file is provided. Futhermore, we provide the database (uniprot-paenibacillus+larvae.fasta) used for the identification of P. larvae proteins created of different strains of P. larvae. The sequences were downloaded of UniProt on 12.03.2019. Finally, the compressed (zipped) folder "combined" is provided. The data were analyzed in MaxQuant v1.6.3.4. [doi:10.25345/C5SP8K] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Paenibacillus larvae ; Paenibacillus larvae subsp. larvae ; Paenibacillus larvae subsp. pulvifaciens ; American foulbrood ; pathogen ; honey bee ; Apis mellifera ; exoproteins ; virulence factors

Contact

Principal Investigators:
(in alphabetical order) 		Tomas Erban, Crop Research Institute, Czechia
Submitting User: arachnid

Publications

Erban T, Zitek J, Bodrinova M, Talacko P, Bartos M, Hrabak J.
Comprehensive proteomic analysis of exoproteins expressed by ERIC I, II, III and IV Paenibacillus larvae genotypes reveals a wide range of virulence factors.
Virulence. 2019 Dec;10(1):363-375.

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