GSC 8-11 cells (20 million) were transduced with lentivirus coding for shACADM 4, 6, or scr (control). ACADM is an enzyme responsible for the metabolism of medium chain fatty acids (C4-C12). A round of selection was performed using puromycin to ensure only cells that had been transduced survived. Knockdown of ACADM was confirmed via western blot. Pellets were collected by centrifuging cells for 10 min at max speed. The pellets were washed and each sample was split into quadruplicate.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Metabolomics Workbench ; ST000819 ; MS analysis
Principal Investigators: (in alphabetical order) |
Maureen Kachman, University of Michigan, N/A |
Submitting User: | ccms |
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