MassIVE MSV000089463

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Segal_TMT_14-3-3_APMS_BioID_interactors

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Description

This dataset consists of 36 raw MS files acquired on Lumos Fusion Orbitrap mass spectrometer operated in Data Dependent Acquisition mode for TMT-10plex. The files are associated with a manuscript submitted for publication by Segal et al. that determines the protein-protein and proximity interaction landscape of 14-3-3 proteins. All samples comprise either BioID or FLAG AP-MS purifications of each of the human 14-3-3 proteins (with negative controls) expressed to the same level in HEK293 Flp-In T-REx cells. Purified samples were labeled with TMT reagents, and analyzed by mass spectrometry. 32 files correspond to the BioID dataset (fractionated; MS3), and 4 files to the AP-MS dataset (non fractionated, HCD MS2). Samples were generated by Dmitri Segal and Stefan Maier and affinity purification, labeling, mass spectrometry, and data analysis were performed by Stefan Maier. Corresponding authors: Mikko Taipale (mikko.taipale@utoronto.ca) and Anne-Claude Gingras (gingras@lunenfeld.ca). Anne-Claude Gingras should be contacted for questions on this dataset. [doi:10.25345/C5348GK9M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Affinity purification ; BioID ; Proximity-dependent biotinylation ; Protein-protein interaction ; AP-MS ; 14-3-3 ; Phosphorylation

Contact

Principal Investigators:
(in alphabetical order) 		Anne-Claude Gingras, LTRI, Canada
Submitting User: gingraslab
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.