This data set contains the following:
1) 66 raw files for NeuCode-labeled human proteoforms from the Jurkat cell line analyzed via "intact-mass" (i.e., LC-MS, with no precursor fragmentation). Three biological replicates were performed (files from the different replicates are labeled with 022317, 031617, or 031817, respectively). For each replicate, proteoforms were separated offline using a 12% Tris-acetate Gelfree cartridge and 11 fractions were collected. Two technical replicate LC-MS injections of each fraction were performed, yielding a total of 66 raw files (3 biological replicates x 11 fractions x 2 injections).
2) 22 raw files for label-free human proteoforms from the Jurkat cell line analyzed via "top-down" (i.e., LC-MS/MS, with precursor fragmentation). One biological replicate was performed (labeled 032017). Proteoforms were separated offline using a 12% Tris-acetate Gelfree cartridge and 11 fractions were collected. Two technical replicate LC-MS/MS injections of each fraction were performed, yielding a total of 22 raw files (1 biological replicate x 11 fractions x 2 injections).
3) Multi-protease and trypsin-only pruned G-PTM-D databases used for proteoform identification. Note that the bottom-up data used to generate these databases can be found elsewhere on MassIVE (MSV000083304).
[doi:10.25345/C5JP71]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: human proteoform, proteoform family, intact-mass, top-down, global PTM discovery, NeuCode
Principal Investigators: (in alphabetical order) |
Lloyd M. Smith, University of Wisconsin-Madison, United States |
Submitting User: | kbuxton |
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Owner | Reanalyses | |
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Experimental Design | ||
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Identification Results | ||
Proteins (Human, Remapped):
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Quantification Results | ||
Differential Proteins:
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Quantified Proteins:
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Browse Dataset Files | |
FTP Download Link (click to copy):
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