MassIVE MSV000093036

Partial Public

PANX1 interacting proteins from Neuro2a cells

Description

Pannexin 1 (PANX1) interacting proteins were identified from mouse Neuro2a cells in the Swayne Lab at the University of Victoria. Proteins co-precipitating with PANX1EGFP or EGFP control were identified by the University of Victoria Genome BC Proteomics Centre. Sample Processing and Data Processing: The University of Victoria Genome BC Proteomics Centre used in-solution trypsin digestion followed by LC-MS/MS. Raw files were analysed with Proteome Discoverer 1.3.0.339 software suite (ThermoScientific). Full details can be found in the PhD thesis of Leigh Wicki-Stordeur at https://dspace.library.uvic.ca/handle/1828/6938. Files: Please consult the file guide for important additional information related to the RAW files. [doi:10.25345/C5QN5ZN7Z] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: pannexin 1, Mus musculus, Neuro2a, interactome

Contact

Principal Investigators:
(in alphabetical order)
Leigh Anne Swayne, University of Victoria, Canada
Submitting User: lswayne
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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.