MassIVE MSV000092716

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GNPS - Multi-omics Characterization of NIST Seafood Reference Materials

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Description

The National Institute of Standards and Technology (NIST) has prepared four seafood reference materials (RMs) for use in food safety and nutrition studies: wild-caught and aquacultured salmon (RM 8256 and RM 8257) and wild-caught and aquacultured shrimp (RM 8258 and RM 8259). These materials were characterized using genetic, metabolomic (1H-NMR, nuclear magnetic resonance and LC-HRMS/MS, liquid chromatography high resolution tandem mass spectrometry), lipidomic and proteomic methods to explore their use as matrix-matched, multi-omic differential materials for method development towards identifying product source and/or as quality control in untargeted omics studies. The results from experimental replicates were reproducible for each reference material and analytical method, with the most abundant features reported. Additionally, differences between the materials could be detected, where wild-caught and aquacultured seafood could be distinguished using untargeted metabolite, lipid and protein analyses. Further processing of the fresh frozen RMs by freeze-drying revealed the freeze-dried seafoods could still be reliably discerned. These results demonstrate the usefulness of these reference materials as tools for omics instrument validation and measurement harmonization in seafood-related studies. Furthermore, their use as differential quality control materials, regardless of preparation method, may also provide a tool for laboratories to demonstrate proficiency at discriminating between products based on source/species. [doi:10.25345/C5XS5JT1K] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: reference material ; seafood ; aquaculture ; wild-caught

Contact

Principal Investigators:
(in alphabetical order) 		Amanda L. Bayless, NIST, USA
Debra L. Ellisor, NIST, USA
Tracey B. Schock, NIST, USA
W. Clay Davis, NIST, USA
Submitting User: clay_davis_NIST

Publications

Ellisor DL, Bayless AL, Schock TB, Davis WC, Knott BT, Seghers J, Leys H, Emteborg H.
Multi-omics characterization of NIST seafood reference materials and alternative matrix preparations.
Anal Bioanal Chem. 2024 Jan;416(3):773-785. Epub 2023 Sep 18.

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GNPS content goes here (MSV000092716 [task=d8d7da5dd9d64bc2a59d42e60dff3a74])
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