MassIVE MSV000084270

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Enrichment of chromatin associated protein in mESC using LC-MS/MS

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Description

Mouse embryonic stem cells lysates were fractionated and separated by mechanical disruption follow by high salt gradient separation in order to obtain chromatin fractionation. Quantitative high mass accuracy mass spectrometry of the whole cell lysate and the chromatin fraction to asses the enrichment of the chromatin associated protein across both samples. [doi:10.25345/C5KM2R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: mESC ; Chromaitn ; Proteomic ; Total cell Lysate

Contact

Principal Investigators:
(in alphabetical order) 		Ole Norregaard Jensen, University of Southern Denmark, SDU, Denmark
Submitting User: oliviero
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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