MassIVE MSV000095301

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Towards a universal method for middle down analysis of antibodies via proton transfer charge reduction Orbitrap mass spectrometry

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Description

We report the development and benchmark of a middle down mass spectrometry strategy based on the combination of different ion fragmentation techniques with proton transfer charge reduction (PTCR) to simplify the gas phase sequencing of mAb subunits. Applied on the liquid chromatography time scale using an Orbitrap Tribrid mass spectrometer, PTCR produces easy to interpret mass spectra with limited ion signal overlap. [doi:10.25345/C59S1KX2Q] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Orbitrap ; middle down ; IgG ; ETD ; HCD ; EThcD ; Orbitrap Eclipse ; proton transfer charge reduction ; synchronous precursor selection ; monoclonal antibody ; Fourier transform mass spectrometry

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Principal Investigators:
(in alphabetical order) 		Luca Fornelli, University of Oklahoma, United States
Submitting User: Fornelli_Lab
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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