The dataset was obtained by performing a TMTpro16plex expression proteomics experiment, where HeLa cells were treated with small molecule ligands of squalene synthase (FDFT1). The experiment was successful in re-producing previously obtained Western Blot results in regards to SQS (=FDFT1) levels and aimed at elucidating downstream effects on cholesterol biosynthesis and metabolism. The dataset comprises data for the following samples: DMSO (vehicle), KY02111, PROTAC 18, SQSI and a compound unrelated to this project (TMTpro16plex, processed by Maxquant, each collected in 30 fractions). The experiments were performed in three independent replicates for each compound (R1-R3).
[doi:10.25345/C5WP9TH8D]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: expression proteomics ; squalene synthase ; FDFT1
Principal Investigators: (in alphabetical order) |
Luca laraia, Technical University of Denmark, Denmark |
Submitting User: | joseho |
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Owner | Reanalyses | |
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