Proteome mapping of Pyrenophora tritici-repentis (Ptr) intracellular proteins
Sample analysis (in brief): - Ptr samples have been concentrated via TCA/acetone protein precipitation - Concentrated samples have been separated into 24 fractions by OFFGEL electrophoresis (IEF) - Proteins of each fraction have then been reduced, alkylated (iodoacetamide) and trypsin-digested - Peptides of each fraction have then been separated on a C18 column in an LC run and analysed by high-resolution mass spectrometry (LTQ Orbitrap XL).
[doi:10.25345/C58C7F]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Fungal pathogen ; Pyrenophora tritici-repentis ; Intracellular proteomics
Principal Investigators: (in alphabetical order) |
Caroline Moffat, Curtin University, Australia |
Submitting User: | paulaM |
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Owner | Reanalyses | |
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Experimental Design | ||
Conditions:
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Proteins (Human, Remapped):
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Proteins (Reported):
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Peptides:
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PSMs:
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Quantification Results | ||
Differential Proteins:
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Quantified Proteins:
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