MassIVE MSV000095714

Partial Public PXD055304

Altering translation allows E. coli to overcome chemically stabilized G-quadruplexes

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Description

Proteomic analysis reveals translation-related proteins are significantly altered in response to stabilized G4s. Identification of the importance of translation factors in overcoming G4s led to the question of how E. coli cells generally respond to chemicals that stabilize such structures. As a first step in addressing this question, a proteomic analysis was carried out to measure the quantitative effects of NMM on the levels of individual proteins in E. coli. Protein levels from early log-phase cultures of delta-tolC and delta-tolC tufA::kan strains grown in the presence or absence of NMM were measured to assess how reduced EF- Tu levels and NMM impacted expression. [doi:10.25345/C5PV6BJ98] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Data-dependent acquisition (DDA) ; Bottom-up proteomics ; E.coli ; G-quadruplex ; Orbitrap Ascend

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Principal Investigators:
(in alphabetical order) 		Joshua J. Coon, University of Wisconsin-Madison, USA
Submitting User: coonlabs
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