MassIVE MSV000085770

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Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

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Description

This is the test data file for a protocol developed to effectively extract intact histones from sorghum leaf materials for profiling of histone post-translational modifications. Histones were extracted from isolated sorghum leaf nuclei and subjected to top down analysis without enzymatic digestion. Data was searched with TopPIC and MSPathFinder. [doi:10.25345/C50T8T] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: epigenetics ; top-down ; mass spectrometry ; plant ; histone ; post-translational modifications

Contact

Principal Investigators:
(in alphabetical order) 		Ljiljana Pasa-Tolic, Pacific Northwest National Laboratory, USA
Submitting User: alchemistmatt

Publications

Mowei Zhou, Shadan H. Abdali, David Dilworth, Lifeng Liu, Benjamin Cole, Neha Malhan, Amir H. Ahkami, Tanya E. Winkler, Joy Hollingsworth, Julie Sievert, Jeff Dahlberg, Robert Hutmacher, Mary Madera, Judith A. Owiti, Kim K. Hixson, Peggy G. Lemaux, Christer Jansson, Ljiljana Paša-Toli?.
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers.
jove. Epub 2021 Mar 04. doi: https://dx.doi.org/10.3791/61707.

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