Description
CE-MS/MS analysis of a pure carcinoembryonic antigen obtained from patient tissue samples. Enzymes used for digestion: trypsin, Glu-C, pronase. CE separation voltage 20 kV, normal polarity. CE-MS/MS parameters: ESI positive mode, glass capillary voltage at 1200 V, drying gas temperature at 150C, drying gas flow rate at 1.2 L/min, nebulizer gas pressure at 0.2 bar, quadrupole ion energy at 3.0 eV and collision cell energy at 7.0 eV. MS data were acquired between m/z 200 and 2000 with a spectral acquisition rate of 1 Hz. MS/MS spectra were acquired in a data dependent mode with an absolute threshold of 4548 counts and active exclusion.
D1 - CEA1 trypsin, D2 - CEA2 trypsin, D3 - CEA3 trypsin, D4 - CEA1 Glu-C, D5 CEA2 Glu-C, D6 - CEA3 Glu-C, D7 - CEA1 pronase, D8 - CEA2 pronase, D9 - CEA3 pronase
[doi:10.25345/C5Z50X]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: glycosylation, carcinoembryonic antigen, colorectal cancer, CE-MS
Contact
Principal Investigators:
(in alphabetical order)
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Guinevere Lageveen-Kammeijer, LUMC, the Netherlands
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| Submitting User: |
VKuzyk
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Conditions:
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Biological Replicates:
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Technical Replicates:
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| Identification Results |
Proteins (Human, Remapped):
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Proteins (Reported):
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Peptides:
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Variant Peptides:
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PSMs:
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Differential Proteins:
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Quantified Proteins:
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Number of distinct conditions across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct condition labels are counted across all files submitted in the "Metadata" category
having a "Condition" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct replicate labels are counted across all files submitted in the "Metadata" category
having a "BioReplicate" or "Replicate" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses)
associated with this dataset.
The technical replicate count is defined as the maximum number of times any one distinct
combination of condition and biological replicate was analyzed across all files submitted in the
"Metadata" category. In the case of fractionated experiments, only the first fraction is
considered.
"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original
submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported
across all analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.