Human foreskin fibroblast cells were spread on fibronectin (FN) for 1 h and treated with DMSO or FAK inhibitor (3 uM, AZ13256675 (termed AZ675 for short)) for 1 h. Integrin-associated adhesion complexes isolated from these cells were analysed by mass spectrometry to determine changes in adhesion complex composition upon FAK inhibition. As a negative control, complexes were also isolated from cells attached to transferrin (Tf), which allows integrin-independent adhesion via the transferrin receptor.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Focal adhesion kinase (FAK) ; integrin ; adhesion ; proteomics ; MS
Principal Investigators: (in alphabetical order) |
Dr Martin J. Humphries |
Submitting User: | ccms |
Horton ER, Humphries JD, Stutchbury B, Jacquemet G, Ballestrem C, Barry ST, Humphries MJ.
Modulation of FAK and Src adhesion signaling occurs independently of adhesion complex composition.
J. Cell Biol. 2016 Feb 1;212(3):349-64.
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