We analyzed non-atherosclerotic repair arteries gathered at coronary by-pass operations from 30 patients with type 2 diabetes, as well as from 30 age- and gender-matched non-diabetic individuals. Quantitative proteome analysis was done by iTRAQ-labelling and LC-MS/MS analysis on individual arterial samples. The amounts of the basement membrane (BM) components, alpha-1- and alpha-2- type IV collagen, gamma-1- and beta-2-laminin were significantly increased in patients with diabetes. Moreover, the expressions of basement membrane components and other vascular proteins were significantly lower among metformin users, as compared to non-users. Patients treated with or without metformin had similar levels of HbA1c, cholesterol and blood pressure. In addition, quantitative histomorphometry showed increased area fractions of collagen-stainable material in tunica intima and media among patients with diabetes.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: type 2 diabetes artery proteomics
Principal Investigators: (in alphabetical order) |
Hans Christian Beck, Odense University Hospital, Sdr Boulevard 29, DK-5000 Odense C, Dept Clinical Biochemistry and Pharmacology, N/A |
Submitting User: | ccms |
Preil SA, Kristensen LP, Beck HC, Jensen PS, Nielsen PS, Steiniche T, Bjørling-Poulsen M, Larsen MR, Hansen ML, Rasmussen LM.
Quantitative Proteome Analysis Reveals Increased Content of Basement Membrane Proteins in Arteries From Patients With Type 2 Diabetes Mellitus and Lower Levels Among Metformin Users.
Circ Cardiovasc Genet. 2015 Oct;8(5):727-35. Epub 2015 Sep 14.
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