Comparison of separation selectivity between 100A and 120A pore size C18 sorbents revealed higher peptide retentivity for the wider-pore packing material, despite it having a smaller surface area. Concurrently, peptide retention shift between 100A and 120A was more pronounced for smaller peptides.
These two findings are at odds with the general notions that (1) larger surface area leads to higher retention, (2) size exclusion effects don't happen for small (<5000 Da) peptides in this pore size range and (3) size exclusion effects are directly (not inversely) proportional to analyte size.
The 100A (col2) and 120A (col3) jurkat digest samples were run in triplicates.
[doi:10.25345/C5PV6BJ6T]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: hplc separation ; proteomics ; fundamental mechanisms
Principal Investigators: (in alphabetical order) |
Oleg Krokhin, University of Manitoba, Canada |
Submitting User: | vspicer1 |
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Owner | Reanalyses | |
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