MassIVE MSV000094098

Partial Public

Mannose-6-phosphate isomerase functional status shapes a rearrangement in the proteome and degradome of mannose-treated melanoma cells

Subscribe Comment Convert Spectra Reanalyze Spectra Add Reanalysis

Description

Metabolic reprogramming is a ubiquitous feature of transformed cells, comprising one of the hallmarks of cancer and enabling neoplastic cells to adapt to new environments. Accumulated evidence reports on the failure of some neoplastic cells to convert mannose-6-phosphate into fructose-6-phosphate, thereby impairing tumor growth in cells displaying low levels of the enzyme mannose-6-phosphate isomerase (MPI). Thus, we performed functional analyses and profiled the proteome landscape and the repertoire of substrates of proteases (degradome) of melanoma cell lines with distinct mutational backgrounds submitted to the treatment with mannose. Our results suggest a significant rearrangement in the proteome and degradome of melanoma cells lines upon mannose treatment, including the activation of catabolic pathways (such as protein turnover) and differences in protein N-terminal acetylation. Such biological implications might reflect the metabolic rewiring caused by the functional status of MPI enzyme and may help target specific molecular players from affected signaling circuits in melanoma. [doi:10.25345/C5R20S715] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Melanoma ; phosphomannose isomerase ; degradomics ; metabolic rewiring ; proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Andre Zelanis, Functional Proteomics Laboratory, Department of Science and Technology, Federal University of Sao Paulo, Sao Jose dos Campos, SP, Brazil, Brazil
Submitting User: azelanis

Publications

Nathália de Vasconcellos Racorti , Matheus Martinelli, Silvina Odete Bustos, Murilo Salardani , Maurício Frota Camacho , Uilla Barcick , Luis Roberto Fonseca Lima , Letícia Dias Lima Jedlicka , Claudia Barbosa Ladeira de Campos , Richard Hemmi Valente , Roger Chammas , André Zelanis.
Mannose-6-Phosphate Isomerase Functional Status Shapes a Rearrangement in the Proteome and Degradome of Mannose-Treated Melanoma Cells.
J Proteome Res. 2024 Oct 18;23(11):5177–5192. doi: 10.1021/acs.jproteome.4c00705.

de Vasconcellos Racorti N, Martinelli M, Bustos SO, Salardani M, Camacho MF, Barcick U, Fonseca Lima LR, Jedlicka LDL, Ladeira de Campos CB, Valente RH, Chammas R, Zelanis A.
Mannose-6-Phosphate Isomerase Functional Status Shapes a Rearrangement in the Proteome and Degradome of Mannose-Treated Melanoma Cells.
J Proteome Res. 2024 Nov 1;23(11):5177-5192. Epub 2024 Oct 18.

Number of Files:
Total Size:
Spectra:
Subscribers:
 
Owner Reanalyses
Experimental Design
    Conditions:
    Biological Replicates:
    Technical Replicates:
 
Identification Results
    Proteins (Human, Remapped):
    Proteins (Reported):
    Peptides:
    Variant Peptides:
    PSMs:
 
Quantification Results
    Differential Proteins:
    Quantified Proteins:
 
Browse Dataset Files
 
FTP Download Link (click to copy):

Species

Instrument

Modifications

- Dataset Reanalyses

+ Dataset History

Click here to queue conversion of this dataset's submitted spectrum files to open formats (e.g. mzML). This process may take some time.

When complete, the converted files will be available in the "ccms_peak" subdirectory of the dataset's FTP space (accessible via the "FTP Download" link to the right).
Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.