The overall goal of this project is to determine the key sites in the C-terminus of eIF6 that are recognized and phosphorylated by GSK3. Analysis of kinase reactions using the site-specific mutants as well as the C-terminus deletion mutants of eIF6 indicated that there are GSK3-specific motifs in the C-terminus that are phosphorylated by GSK3. The purpose of the Mass Spectrometry studies is to use MS as a targeted and secondary approach to validate and identify GSK3-specific sites of phosphorylation in the C-terminus of eIF6.
[doi:10.25345/C5ZX41]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Phosphorylation, eIF6, kinase assay GSK3, priming, immunoprecipitation
Principal Investigators: (in alphabetical order) |
Dr. Sofia Origanti, Saint Louis University, USA |
Submitting User: | gsabat |
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Owner | Reanalyses | |
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