Non-coding RNA (sRNA) are playing a key role in gene expression in bacteria. In general, sRNA regulates gene expression by base-pairing with ribosome binding sites to block translation. The modification of the ribosome trafficking generally affects mRNA stability. However, few examples have been described in bacteria where sRNA binding are mainly affected translation without modifying mRNA stability. In order to identify such targets in B. subtilis, we developed a pulsed-SILAC method allowing to label new protein synthesis after a short expression of the sRNA. We applied it to the best characterized sRNA in this bacteria, namely RoxS. RoxS sRNA was previously shown to interfere with the expression of genes encoding enzymes of the central metabolism to control the NAD/NADH ratio in B. subtilis.
[doi:10.25345/C5CF9JH05]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: pulse SILAC ; RoxS ; small RNA
Principal Investigators: (in alphabetical order) |
Sylvain Durand, CNRS-UMR8261, France |
Submitting User: | Marion_T |
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Owner | Reanalyses | |
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