MassIVE MSV000099384

Partial Public PXD069137

Reconnecting Vagus Nerve to the Heart through Nerve Conduit Preserves Cardiac Function in a Minipig Model of Right Cardiac Vagotomy

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Description

The right vagus nerve (RVN) plays a crucial role in maintaining cardiac homeostasis, and its intrathoracic resection leads to postoperative cardiac complications. Developing effective method to repair the RVN after surgical transection at the heart level is a critical unmet clinical need. Here we show that early reconnection of the RVN to the heart promoted by implantable Chitosan/Poly-e-caprolactone cuff-like nerve guidance conduit (ChiPCL. C-NGC) significantly preserves cardiac mechanical function in minipigs subjected to right cardiac vagotomy (RVT). Animals that were randomly treated with ChiPCL. C-NGC showed improved global circumferential, longitudinal, and radial strain, along with reduced diastolic dyssynchrony. Histopathology revealed 20% viable vagal fascicles, normalized levels of myocardial parasympathetic fibers, oxidative stress- and aging-related markers, and prevented interstitial myocardial fibrosis in treated group. Our findings suggest that preserving a limited number of RVN fascicles and their neurofilaments prevents early cardiac remodeling by lowering oxidative stress-mediated premature senescence of cardiac cells. Reconnecting the RVN to the heart via ChiPCL C-NGC presents a potential therapeutic strategy to prevent RVT-induced heart failure following thoracic surgery or heart and lung transplantation [doi:10.25345/C5JH3DG01] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Vagus Nerve, Vagotomy, Implatation, Aging markers, Minipigs ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Alessandro Cellerino, Leibniz Institute on Aging, Beutenbergstr. 11, 07745 Jena, Germany
Submitting User: ProteomicsCF_FLI
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