MassIVE MSV000094438

Partial Public PXD051092

Single-cell proteomics captures the Proteome Heterogeneity in Human iPSC-Derived Cardiomyocytes and Adult Cardiomyocytes

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Description

Human induced pluripotent stem cell (IPSC)-derived cardiomyocytes (iCMs) have become important tools to model cardiovascular diseases and drug toxicology. Despite suggested transcriptomic heterogeneity in both iPSC and iCMs, the cellular proteome heterogeneity is poorly understood. Using cutting-edge single cell proteomics, we quantify the maturation from IPSC to iCMs and observed two distinct populations of iCMs with different metabolism, which recapitulates the single adult cardiomyocyte proteome populations albeit less mature. [doi:10.25345/C5T727S7Q] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: iPSCs ; CMs ; differentiation ; single cell

Contact

Principal Investigators:
(in alphabetical order) 		Jennifer Van Eyk, Cedars Sinai Medical Center, United States
Submitting User: bineka
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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