Identification of peptides and proteins of 18 different insect meal samples derived from the species Hermetia illucens (8), Tenebrio molitor (5), Alphitobius diaperinus (3) and Acheta domesticus (2). Proteins were extracted using two different extraction and solubilization methods. Protein digests were analyzed using an ESI-MS/MS maXis Impact UHR-TOF (Bruker) coupled with an UltiMate 3000 HPLC system (Thermo Scientific) using a 1 mm I.D. x 150 mm reverse phase column (Acclaim PepMap 100 C18, Thermo). Mass spectrometry data (.baf files) were converted using compassXport (Bruker DataAnalysis 4.2) and saved as mzXML files. Proteomics data analysis was conducted using the proteoQC package (version 1.18.1) in R (version 3.4.4) running in RStudio (version 1.0.143).
[doi:10.25345/C5VH1T]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: insect meal ; processed animal protein
Principal Investigators: (in alphabetical order) |
Josef Daniel Rasinger, Institute of Marine Research, Norway |
Submitting User: | jdrasinger |
Belghit, I., Lock, E.-J., Fumière, O., Lecrenier, M.-C., Renard, P., Dieu, M., Berntssen M.H.G., Palmblad, M., Rasinger, J. D.
Species-Specific Discrimination of Insect Meals for Aquafeeds by Direct Comparison of Tandem Mass Spectra.
Belghit, I., Lock, E.-J., Fumière, O., Lecrenier, M.-C., Renard, P., Dieu, M., … Rasinger, J. D. (2019). Species-Specific Discrimination of Insect Meals for Aquafeeds by Direct Comparison of Tandem Mass Spectra. Animals, 9(5), 222.
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