MassIVE MSV000082363

Complete Public PXD009790

Human chromatin platforms for the study of transcription and RNA regulation

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Description

Here we describe tools to study the step-wise assembly of protein complexes on chromatin in a highly-controlled manner using reconstituted chromatin platforms and quantitative proteomic profiling. We profile the early steps in transcriptional activation and highlight the potential for understanding the multiple ways chromatin can influence transcriptional regulation. We also describe modifications of this approach to study the activity of a long noncoding RNA to act as a dynamic scaffold for proteins to be recruited to chromatin. The reconstituted nature of the chromatin substrate offers a tune-able system, able to be trapped at specific sub-steps, to understand how chromatin interfaces with genome regulation machinery. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: SILAC chromatin RNA transcription

Contact

Principal Investigators:
(in alphabetical order) 		Aaron Johnson, University of Colorado Denver, USA
Submitting User: kirkhansen

Publications

Balas MM, Porman AM, Hansen KC, Johnson AM.
SILAC-MS profiling of reconstituted human chromatin platforms for the study of transcription and RNA regulation.
J Proteome Res. 2018 Sep 7. doi: 10.1021/acs.jproteome.8b00395. [Epub ahead of print].

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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