MassIVE MSV000087992

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Child et al SRPR Data Set_SILAC_TMT

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Description

Here we utilized CRISPR/Cas9 editing and RNAi to disable SR protein expression in HeLa cells. Loss of SR heterodimer expression was confirmed by SILAC proteomic analyses in HeLa Cas9 parental and SR-deficient cells. These studies also demonstrated that the membrane protein proteome was largely unaltered by loss of SR, though reduced expression of a limited subset of membrane proteins was observed. [doi:10.25345/C5V54H] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: SILAC ; TMT ; Subcellular fractionation ; cell homogenization ; carbonate extraction ; integral membrane proteins ; signal recognition particle receptor

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Principal Investigators:
(in alphabetical order) 		Dr. Christopher Nicchitta, Duke University, United States of America
Submitting User: NicchittaLab
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