MassIVE MSV000084374

Imported Reanalysis Dataset Public PXD010162

Investigation of cytoplasmic cGAS interactions reveals association with OASL during HSV-1 infection

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Description

Viral DNA sensing is an essential component of mammalian innate immune response. Upon binding viral DNA, the cyclic-GMP-AMP synthase (cGAS) catalyzes the production of cyclic dinucleotides to induce type I interferons. However, little is known about how cGAS is homeostatically maintained or regulated upon infection. Here, we define cytoplasmic cGAS interactions with cellular and viral proteins upon herpes simplex virus (HSV-1) infection in primary human fibroblasts. We compare several HSV-1 strains (wild-type, d109, d106) that induce cytokine responses and apoptosis, and place cGAS interactions in the context of temporal proteome alterations using isobaric-labeling mass spectrometry. Follow-up analyses establish a functional interaction between cGAS and 2â??-5â??-oligoadenylate synthase-like protein OASL. The OAS-like domain interacts with the cGAS Mab21 domain, while the OASL ubiquitin-like domain further inhibits cGAS-mediated interferon response. Our findings explain how cGAS may be inactively maintained in cellular homeostasis, with OASL functioning as a negative feedback loop for cytokine induction. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: cGAS ; IP-MS ; TMT ; OASL ; interactions ; DNA sensing

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Principal Investigators:
(in alphabetical order) 		Ileana M Cristea, Department of Molecular Biology, Princeton University, USA, N/A
Submitting User: ccms
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