MassIVE MSV000089171

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Organelle resolved proteomics reveals new chordoma cell surface markers required for proliferation and association with outcome

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Description

Here, we used a proteomics approach to identify novel chordoma-specific cell-surface protein markers. Four established chordoma cell lines (U-CH17P, U-CH17M, U-CH17S and U-CH11R) were analyzed by quantitative proteomics using a comprehensive organellar fractionation approach based on differential ultracentrifugation. A subtractive proteomics strategy was applied to identify proteins that are plasma membrane enriched. The expression profiles of these cell-surface proteins were validated across chordoma cell lines, patient surgical tissue samples, and normal tissue lysates. The essentiality of these candidates was evaluated using chordoma cell line growth in vitro. [doi:10.25345/C5BC3T179] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Chordoma ; Proteomics ; Organelle fractionation ; Cell-surface proteins

Contact

Principal Investigators:
(in alphabetical order) 		Dr. Thomas Kislinger, Princess Margaret Cancer Centre, Canada
Submitting User: TKislinger

Publications

Khan S, Zuccato JA, Ignatchenko V, Singh O, Govindarajan M, Waas M, Mejia-Guerrero S, Gao A, Zadeh G, Kislinger T.
Organelle resolved proteomics uncovers PLA2R1 as a novel cell surface marker required for chordoma growth.
Acta Neuropathol Commun. 2024 Mar 7;12(1):39. Epub 2024 Mar 7.

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