Chinese Hamster Ovary (CHO) cells are frequently used to produce recombinant HIV envelope protein gp120. In this study, we characterized the CHO proteins that become co-purified with gp120 when lectin affinity chromatography is used. Many of co-purified CHO proteins identified were extracellular matrix components. We verified a method for separating gp120 from these CHO proteins with anion exchange chromatography, and assessed the biochemical activity of gp120 preparations with and without co-purified CHO proteins.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: HIV ; gp120 ; fibronectin ; extracellular matrix ; CHO ; Chinese Hamster
Principal Investigators: (in alphabetical order) |
Shiu-Lok Hu, University of Washington, N/A |
Submitting User: | ccms |
Plotnik D, Guo W, Cleveland B, von Haller P, Eng JK, Guttman M, Lee KK, Arthos J, Hu SL.
Extracellular Matrix Proteins Mediate HIV-1 gp120 Interactions with ?4?7.
J. Virol. Epub 2017 Oct 13.
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