MassIVE MSV000099281

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Untargeted Lipidomics of Liver and Plasma of Plin4 Knockout and Wild-Type Mice Fed Control or Western Diet

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Description

This study investigates the impact of Plin4 gene deletion on lipid metabolism in mouse liver and plasma under different dietary conditions using untargeted lipidomics. Female wild-type (Plin4+/+) and knockout (Plin4-/-) mice were fed either a control diet or a Western diet high in fat, fructose, and cholesterol. Samples included liver tissue and plasma (n = 5 per tissue type per group, total n = 40). Lipids were extracted using isopropanol, with pooled quality control samples prepared and injected regularly for signal correction. Lipidomic analysis was performed using ultra-high-performance liquid chromatography coupled to Orbitrap mass spectrometry (UHPLC-MS), with data acquired using Xcalibur and processed in Compound Discoverer and LipidSearch. Features were normalized using the SERRF algorithm, and lipid identifications were based on MS/MS fragmentation matching. This dataset provides global lipid profiles for evaluating the metabolic effects of Plin4 deletion in both normal and diet-induced obese states. [doi:10.25345/C5VD6PJ1M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: lipidomics ; liver ; plasma ; mice ; control diet ; western diet ; plin4 knockout ; plin4 wild type ; DatasetType:Metabolomics

Contact

Principal Investigators:
(in alphabetical order) 		Knut Tomas Dalen, Department of Nutrition, Institute of Basic Medical Science, Faculty of Medicine, University of Oslo, Norway
Submitting User: AlesKvasnicka

Publications

Doncheva AI, Higa R, Khanal P, Øksenvåg Ingebrigtsen MV, Li Y, Kolan S, Kolan P, Kvasnicka A, Jermstad I, Khan S, Skålhegg BS, Kolset SO, Nebb H, Hjorth M, Norheim FA, Dalen KT.
Elevated adipose inflammation, but reduced hepatic triacylglycerol storage in diet-induced obese Plin4-/- mice.
J Biol Chem. Epub 2025 Dec 12.

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