MassIVE MSV000090855

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Alkynyl nicotinamides with antileukemic activity to treat poor prognosis AM

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Description

FLT3 activating mutations cause myeloproliferative neoplasms by deregulating hematopoietic progenitor cell growth, and acute myeloid leukemia is on the rise. Investigational drugs targeting mutant FLT3, including Quizartinib and Crenolanib, develop inherent and acquired resistance to FLT3 targeted therapy. FLT3 inhibitor resistance in AML is dependent on co-occurring mutations, parallel survival pathways, and/or subsequent FLT3-ITD mutations. Despite the high prevalence of FLT3 mutations and their clinical significance in AML, there are few targeted therapeutic options. We identified two novel naphthyridine-based FLT3 inhibitors (HSN608 and HSN748) that specifically target FLT3-ITD at sub-nanomolar concentrations and are effective against drug-resistant secondary mutations. In the current study, we evaluated these compounds antileukemic activity against FLT3-ITD and gatekeeper mutations in drug-resistant AML, relapsed/refractory AMLs with FLT3 mutations, and in vivo mouse models with combinations of epigenetic mutations TET2 with FLT3-ITD, and AML patients PDX. In these model systems, we demonstrate that HSN748 outperforms the FDA-approved FLT3 inhibitor Gilteritinib in terms of inhibitory activity against FLT3-ITD. [doi:10.25345/C5DJ58N0G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: acute myeloid leukemia ; FLT3 ; proteomics ; mass spectrometry ; FLT3 inhibitor

Contact

Principal Investigators:
(in alphabetical order) 		Dr. Herman O. Sintim, Purdue University, USA
Submitting User: uma_aryal
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