MassIVE MSV000095457

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Polyomavirus ALTOs, but not MTs, downregulate viral early gene expression by activating the NF-KB pathway

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Description

Polyomaviruses are small, circular dsDNA viruses that can cause cancer. Alternative splicing of polyomavirus early transcripts generates large and small tumor antigens (LT, ST) that play essential roles in viral replication and tumorigenesis. Some polyomaviruses also express middle tumor antigens (MTs) or alternate LT open reading frames (ALTOs), which are evolutionarily related but have distinct gene structures. MTs are a splice variant of the early transcript whereas ALTOs are overprinted on the second exon of the LT transcript in an alternate reading frame and are translated via an alternative start codon. Merkel cell polyomavirus (MCPyV), the only human polyomavirus that causes cancer, encodes an ALTO but its role in the viral lifecycle and tumorigenesis has remained elusive. In this MassIVE entry, we provide mass spectrometry data and result files associated with a TurboID experiment to identify human and viral proteins that bind to ALTO. [doi:10.25345/C5599ZD0J] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: label free quantification, LFQ, Merkel cell carcinoma, ALTO

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Principal Investigators:
(in alphabetical order) 		Denise Galloway, Fred Hutchinson Cancer Center, United States
Submitting User: FHproteomics
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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