MassIVE MSV000095609

Partial Public PXD054913

Binding proteins of Hydroxymethylcytosine-modified CpG dyads

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Description

Cytosine (C) modifications within CpG dyads are central regulatory elements of mammalian genomes. In addition to 5-methylcytosine (mC), genomes can also contain high levels of 5-hydroxymethylcytosine (hmC) that is read differently by nuclear proteins than mC. However, hmC can co-exist with C and mC in different combinations in the two strands of CpG dyads, and it is poorly understood, how these combinatorial marks differ in their protein interactomes and regulatory functions. To identify nuclear binding proteins, we employed DNA promoter probes with differentially modified CpG dyads in pulldown experiments. The datasets comprise three biological replicates of HEK293T nuclear proteins binding to a VEGFA promoter sequence with differentially modified CpG dyads. Each biological replicate contains five technical replicates per modified CpG dyad, resulting in a total of 15 replicates for C/C, mC/mC and hmC/mC, and 10 replicates for hmC/C and hmC/hmC. [doi:10.25345/C5D21RW5G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Cytosine modifications, nuclear binding proteins, binding to VEGFA promoter, interaction proteomics, bottom-up proteomics

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Principal Investigators:
(in alphabetical order) 		Petra Janning, MPI of Molecular Physiology, Germany
Submitting User: janning
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