MassIVE MSV000101844

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Oxidative stress stimulates Paneth cell plasticity and suppresses antimicrobial function

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Description

Paneth cells are known to metabolically support intestinal stem cells in the small intestine through active lactate production. In our recent research, we found that oxidative stress induces Paneth cell plasticity. However, during this process, it remains unclear whether Paneth cells continue producing lactate and how oxidative stress affects Paneth cell metabolism. To address this question, we cultured small intestinal organoids derived from our Paneth cell reporter mice, in which Paneth cells are labeled with tdTomato following 4-OHT treatment. Day 4 organoids pretreated with 4-OHT were treated with PBS, 1 uM FCCP, or 1 mM H2O2. Twenty-four hours after treatment, the reagents were washed out, and 17.5 mM D-glucose (U-13C6) was added to the organoid culture medium for isotope labeling. After 17 hours of labeling, organoids from each treatment group were dissociated into single cells and sorted into Paneth cells (tdT+) and non-Paneth cells (tdT-) by flow cytometry. Metabolites were then extracted from the sorted cells and subjected to negative-mode polar metabolomics analysis. [doi:10.25345/C5RJ4984M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Paneth cell, lactate, glycolysis, OXPHOS ; DatasetType:Metabolomics

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Principal Investigators:
(in alphabetical order) 		Nan Gao, Rutgers University, Newark, USA
Submitting User: naiduccmb
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