MassIVE MSV000092011

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A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant

Description

Green leaf volatiles (GLVs) are short-chain oxylipins that are emitted from plants in response to stress. Previous studies have shown that oral secretions (OS) of the tobacco hornworm Manduca sexta, introduced into plant wounds during feeding, catalyze the re-arrangement of GLVs from Z-3- to E-2-isomers. This change in the volatile signal however is bittersweet for the insect as it can be used by their natural enemies, as prey location cue. Here we identified a (3Z):(2E)-hexenal isomerase (Hi-1) in M. sexta OS that catalyzes the conversion of the GLV Z-3-hexenal to E-2-hexenal. Hi-1 mutants that were raised on a GLV27 free diet showed developmental disorders, indicating that Hi-1 also metabolizes other substrates important for the insects development. Phylogenetic analysis placed Hi-1 within the GMCb-subfamily and showed that Hi-1 homologs from other lepidopterans could catalyze similar reactions. Our results indicate that Hi- 1 not only modulates the plants GLV-bouquet but also functions in insect development. [doi:10.25345/C5FF3M92M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: In gel digest ; QTOF-1 ; Manduca sexta ; Green leaf volatiles ; GMC oxidoreductase

Contact

Principal Investigators:
(in alphabetical order)
Gertjan Kramer, Laboratory for Mass Spectrometry of Biomolecules, Netherlands
Submitting User: gkramer

Publications

Lin YH, Silven JJM, Wybouw N, Fandino RA, Dekker HL, Vogel H, Wu YL, de Koster C, Große-Wilde E, Haring MA, Schuurink RC, Allmann S.
A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant.
Nat Commun. 2023 Jun 28;14(1):3666. Epub 2023 Jun 28.

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