MassIVE MSV000082752

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A viral Argonaute suppressor implements a dual program to block antiviral immunity

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Description

RAW files accompanying the PPI data in our 2018 Cell Host Microbe manuscript. AP-MS experiments exploring host interactions of the 1A viral suppressor proteins from DCV and CRPV along with mechanistically related experiments (see manuscript for details). Each file corresponds to one gel band from geLC-MS analysis of His-Flag tandem affinity purified bait proteins stably expressed in Drosophila Melanogaster S2 cell line. Each purification was run by SDS-PAGE and the entire lane was cut into 6 gel bands. 3-5 replicate purifications were performed. Five negative control purifications were performed: 2 purifications from untransfected S2 cells, and 3 purifications from tagged eGFP. The data were analyzed by looking at enrichment over the 3 control purifications with highest spectral counts for a given prey using SAINT express. A key mapping experiment to RAW file is provided as an excel file. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: protein interactions, host-pathogen interactions

Contact

Principal Investigators:
(in alphabetical order) 		Raul Andino, University of California San Francisco, USA
Submitting User: mtrnka

Publications

Arabinda Nayak, Dong Young Kim, Michael J Trnka, Craig H Kerr, Peter V Lidsky, David J Stanley, Brianna Monique Rivera, Kathy H Li, Alma L Burlingame, Eric Jan, Judith Frydman, John D Gross, Raul Andino.
A viral Argonaute suppressor implements a dual program to restrict antiviral immunity.
Cell Host Microbe in press.

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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.