The role of protein turnover in pancreatic ductal adenocarcinoma (PDA) metastasis has not previously been investigated. We introduce dynamic Stable-Isotope Labeling of Organoids (dSILO): a dynamic SILAC derivative that combines a pulse of isotopically labeled amino acids with isobaric tandem-mass tag (TMT) labeling to measure proteome-wide protein turnover rates in organoid systems.
[doi:10.25345/C5C24QZ1V]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: SILAC ; PDA ; dSILO ; protein turnover ; protein half-life ; metastases ; proteome turnover
Principal Investigators: (in alphabetical order) |
Christine Chio, Columbia University Irving Medical Center, United states Marko Jovanovic, Columbia University, USA |
Submitting User: | mj2794 |
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Owner | Reanalyses | |
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